A novel ratiometric fluorescence nanoprobe based on aggregation-induced emission of silver nanoclusters for the label-free detection of biothiols

作者：Zhou, Jie; Xiao, Yan^*; Zhang, Xiuhua^*; Wang, Shengfu

来源：Talanta, 2018, 188: 623-629.

DOI：10.1016/j.talanta.2018.06.028

• Aggregation-induced emission
• Silver nanoclusters
• g-C3N4 nanosheets
• Ratiometric fluorescence nanoprobe
• Biothiols

In this work, a novel ratiometric fluorescence nanoprobe based on Au3+-triggered aggregation-induced emission (AIE) behavior of silver nanoclusters (Ag NCs) was designed for the label-free detection of biothiols. The probe was constructed by loading of Au3+ on g-C3N4 nanosheets surface and subsequently aggregating Ag NCs via ion binding. As Au3+ could conversely regulate the emission of g-C3N4 nanosheets and Ag NCs, the remove of Au3+ from nanoprobe by coordination with biothiols would change the emission ratio of nanoprobe that could be used for biothiols detection. The probe provided high sensitivity for glutathione (GSH) determination with the limit of detection as low as 0.8 mu M and showed satisfying performance in human serum samples. This report may offer a new sight for the construction of ratiometric probe based on the AIE behavior of Ag NCs and broaden its applications in biosensing.

• 出版日期2018-10-1
• 单位湖北大学