Multiplexed detection of microRNA biomarkers from tumor cells and tissues with a homogeneous nano-photon switch

作者：Huang, Ru; Liao, Yuhui; Zhou, Xiaoming^*; Fu, Yu; Xing, Da^*

来源：Sensors and Actuators B: Chemical , 2017, 247: 505-513.

DOI：10.1016/j.snb.2017.03.055

• MicroRNA
• Graphene oxide
• Quantum dots
• Enzyme-free amplification
• Tumor molecular diagnosis

MicroRNAs (miRNAs) have been served as the promising biomarkers for tumor diagnosis. Generally, miRNAs undergo synergistic adjustment in tumor cells, for instance, both miRNA21 and miRNA155 are highly expressed in breast cancer cells. Thus, the combinational detection of multiple miRNAs has practical significance. In this study, we constructed a homogeneous nano-photon switch based on quantum dots (QDs) and graphene oxide (GO) for the combinational detection of multiple miRNAs in tumor cells and tissues. Moreover, multiple enzyme-free DNA circuits were constructed to perform the function of signal amplification, and the homogeneous nano-photon switch could emit characteristic signals corresponding to specific miRNAs. The results of miRNA detection from tumor cells indicated that this platform realized the high sensitivity of 10(2) cells. Its excellent performance in tumor tissue also confirmed this platform as a potentially reliable, multivariate assay that could reduce the false-negative and false-dismissal rates of routine tests.

• 出版日期2017-8
• 单位华南师范大学