摘要

The broad applications of laser technique in polymerase chain reaction (PCR) microfluidic chips have been reviewed, including its applications onto microfabrication of PCR microfluidic chips, measures of microfluidic physical parameters and controls of PCR temperature cycling, as well as on-chip PCR product detection( for example fluorescence real-time/end-point detection and capillary electrophoresis (CE detection). Finally, the bright future of laser application in the PCR-based micro-total analysis system (mu TAS) was prospected.