siRNA as a molecular tool for use in Aspergillus niger

作者：Barnes Sally E; Alcocer Marcos J C; Archer David B^*

来源：Biotechnology Letters, 2008, 30(5): 885-890.

DOI：10.1007/s10529-007-9614-0

• Aspergillus niger
• down-regulation
• GUS
• protoplasts
• siRNA

Gene silencing using siRNA has been examined in the industrially-important fungus, Aspergillus niger. Protoplasts of an A. niger strain containing a single genomic copy of the Escherichia coli uidA gene, encoding beta-glucuronidase (GUS), under control of the A. niger glaA promoter at the same genomic locus, were exposed to siRNA targeted against the uidA gene. Down-regulation of uidA mRNA and GUS activity by siRNA was observed in mycelia that developed from the protoplasts. The down-regulation was transient and was not carried over to conidiation. We concluded that gene silencing by siRNA provides a relatively quick method for analysis of gene function in A. niger.

• 出版日期2008-5