Nitro-oleic acid decreases transcription of the angiotensin II type I receptor gene in aortic smooth muscle cells

作者：Wang Huan^*; Ouyang Hongsheng; Tian Yaping; Li Zhuang; Han Xiaolei; Liu Xingxing; Ran Guangyao; Wang Gangqi; Pang Daxin; Tang Xiaochun

来源：Biotechnology and Bioprocess Engineering, 2014, 19(4): 740-746.

DOI：10.1007/s12257-014-0255-6

• nitroalkenes
• angiotensin II type 1 receptor
• smooth muscle cells
• transcriptional regulation

Nitroalkene derivatives of nitro-oleic acid (OA-NO2) regulate pluripotent cell signaling in vivo under physiological and pathological conditions. Angiotensin II type 1 receptor (AT(1)R) plays an important role in the cardiovascular system. In this study, OA-NO2 reduced the AT(1)R mRNA level, specifically in primary smooth muscle cells, showing a 70% reduction in rat smooth muscle cells (RASMCs) and a 50% reduction in pig smooth muscle cells (PASMCs). These effects were not observed in CHO cells, which highly express AT(1)R. The AT(1)R mRNA decay rate was unchanged after OA-NO2 compared with OA treatment. Nitric oxide (NO) and peroxisome proliferator-activated receptor gamma (PPAR gamma) did not alter the reduced effects of OA-NO2 on the AT(1)R mRNA level in SMCs. However, Sp1-mediated activation of the AT(1)R promoter was reduced in response to OA-NO2 in RASMCs but not 293T cells. In addition, the nuclear factor-kappa B (NF-kappa B) pathway was involved in the OA-NO2-mediated downregulation of AT(1)R transcription in SMCs. Taken together, our results demonstrate that OA-NO2 specifically inhibits AT(1)R mRNA expression in primary smooth muscle cells via the NF-kappa B pathway.

• 出版日期2014-7
• 单位吉林农业科技学院; 吉林大学