科研之友
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摘要
Accumulating evidence has revealed that a natural compound piceatannol exerts its anti-tumor activity in a variety of tumors cancer. In the current study, we explored the potential anti-pancreatic cancer activity of piceatannol in vitro, and studied the underlying mechanisms. Cells viability was evaluated using cell counting Kit-8. Apoptosis were analyzed by flow cytometry. Bcl-2, Bax and caspase-3 levels were analyzed by Western blot and miR-125b levels were determined by real-time RT-PCR. The effects of miR-125b on pancreatic cancer cells were assessed by silencing and over-expressing the miRNA in vitro. Our results indicated that piceatannol significantly inhibited PC cells growth while inducing considerable cell apoptosisthrough up-regulating the expression of miR-125b, and inhibiting expression of Bcl-2. Moreover, overexpression of miR-125b in pancreatic cancer could promote the cell apoptosis of PANC-1 and SW1990 cells. Downregulation of miR-125b not only alleviates the reduction of piceatannol on PANC-1 and SW1990 cells, but also promoted expression of Bcl-2 that was suppressed by piceatannol. Therefore, our findings would provide a new insight into the use of piceatannol against pancreatic cancer in future.
- 出版日期2017
- 单位上海交通大学; 上海市闵行区中心医院
