An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles

作者：Subramanian Palaniappan; Motorina Anastasiia; Yeap Weng Siang; Haenen Ken; Coffinier Yannick; Zaitsev Vladimir; Niedziolka Jonsson Joanna; Boukherroub Rabah; Szunerits Sabine^*

来源：Analyst, 2014, 139(7): 1726-1731.

DOI：10.1039/c3an02045b

Nanostructured boron-doped diamond has been investigated as a sensitive impedimetric electrode for the detection of immunoglobulin G (IgG). The immunosensor was constructed in a three-step process: (i) reactive ion etching of flat boron-doped diamond (BDD) interfaces to synthesize BDD nanowires (BDD NWs), (ii) electrochemical deposition of nickel nanoparticles (Ni NPs) on the BDD NWs, and (iii) immobilization of biotin-tagged anti-IgG onto the Ni NPs. Electrochemical impedance spectroscopy (EIS) was used to follow the binding of IgG at different concentrations without the use of any additional label. A detection limit of 0.3 ng mL(-1) (2 nM) with a dynamic range up to 300 ng mL(-1) (2 mu M) was obtained with the interface. Moreover, the study demonstrated that this immunosensor exhibits good stability over time and allows regeneration by incubation in ethylenediaminetetraacetic acid (EDTA) aqueous solution.

• 出版日期2014