Analysis of Volatile Organic Compounds in Human Saliva by a Static Sorptive Extraction Method and Gas Chromatography-Mass Spectrometry

作者：Soini Helena A; Klouckova Iveta; Wiesler Donald; Oberzaucher Elisabeth; Grammer Karl; Dixon Sarah J; Xu Yun; Brereton Richard G; Penn Dustin J; Novotny Milos V^*

来源：Journal of Chemical Ecology, 2010, 36(9): 1035-1042.

DOI：10.1007/s10886-010-9846-7

• Chemical communication
• Gas chromatography-mass spectrometry (GC-MS)
• Human saliva
• Stir bar extraction
• Volatile organic compounds

Human saliva not only helps control oral health (with anti-microbial proteins), but it may also play a role in chemical communication. As is the case with other mammalian species, human saliva contains peptides, proteins, and numerous volatile organic compounds (VOCs). A high-throughput analytical method is described for profiling a large number of saliva samples to screen the profiles of VOCs. Saliva samples were collected in a non-stimulated fashion. The method utilized static stir bar extraction followed by gas chromatography-mass spectrometry (GC-MS). The method provided excellent reproducibility for a wide range of salivary compounds, including alcohols, aldehydes, ketones, carboxylic acids, esters, amines, amides, lactones, and hydrocarbons. Furthermore, substantial overlap of salivary VOCs and the previously reported skin VOCs in the same subject group was found in this study by using pattern recognition analyses. Sensitivity, precision, and reproducibility of the method suggest that this technique has potential in physiological, metabolomic, pharmacokinetic, forensic, and toxicological studies of small organic compounds where a large number of human saliva samples are involved.

• 出版日期2010-9