Anthelmintic constituents from ginger (Zingiber officinale) against Hymenolepis nana

作者：Lin Rong Jyh; Chen Chung Yi; Lu Chin Mei; Ma Yi Hsuan; Chung Li Yu; Wang Jiun Jye; Lee June Der; Yen Chuan Min^*

来源：Acta Tropica, 2014, 140: 50-60.

DOI：10.1016/j.actatropica.2014.07.009

• Cestocidal activity
• Hymenolepis nana
• Ginger
• Anthelmintic activity
• Oxygen radical absorbing capacity
• Cytokine

This study investigated the anthelmintic activity of gingerenone A, [6]-dehydrogingerdione, [4]-shogaol, 5-hydroxy-[6]-gingerol, [6]-shogaol, [6]-gingerol, [10]-shogaol, [10]-gingerol, hexahydrocurcumin, 3R,5S-[6]-gingerdiol and 35,55-[6]-gingerdiol, a constituent isolate from the roots of ginger, for the parasite Hymenolepis nano. The cestocidal activity or ability to halt spontaneous parasite movement (oscillation/peristalsis) in H. nana of above constituents was reached from 24 to 72 h in a time- and dose-dependent manner, respectively. The [10]-shogaol and [10]-gingerol have maximum lethal efficacy and loss of spontaneous movement than the others at 24-72 h. In addition, worms treated with 1 and 10 mu M [10]-gingerol, more than 30% had spontaneous movement of oscillation at 72 h but [10]-shogaol at 72 h only about 15-20% of oscillation. This showing that [10]-gingerol had less loss of spontaneous movement efficacy than [10]-shogaol. After exposure to 200 mu M [10]-shogaol, 100% of H. nano had died at 12 h rather than died at 24h for [10]-gingerol, showing that [10]-gingerol had less lethal efficacy than [10]shogaol. In addition, these constituents of ginger showed effects against peroxyl radical under cestocidal activity. In order to evaluate the cestocidal activity and cytokine production caused by ginger%26apos;s extract RO in the H. nana infected mice, we carried out in vivo examination about H. nano infected mice BALB/c mice were inoculated orally with 500 eggs. After post-inoculation, R0 (1 g/kg/day) was administered orally for 10 days. The RU exhibited cestocidal activity in vivo of significantly reduced worms number and cytokines production by in vitro Con A-stimulated spleen cells showed that INF-gamma and IL-2 were significantly increases by RO. IL-4, IL-5, IL-6, IL-10 and IL-13 were significantly decreases and Murine KC and IL-12 were not significantly changes by R0. Together, these findings first suggest that these constituents of ginger might be used as cestocidal agents against H. nano.

• 出版日期2014-12
• 单位河北医科大学