Dispersive liquid-liquid microextraction of organophosphorous pesticides using nonhalogenated solvents

作者：Henriques Alves Andreia Cristina; Pontes Boavida Goncalves Maria Margarida; Serrano Bernardo Maria Manuel; Mendes Benilde Simoes

来源：Journal of Separation Science, 2012, 35(19): 2653-2658.

DOI：10.1002/jssc.201200241

• Nonhalogenated solvents
• Dispersive liquid-liquid microextraction
• Gas chromatography-mass spectrometry
• Organophosphorous pesticides

Dispersive liquidliquid microextraction (DLLME) combined with gas chromatography and mass spectrometry (GC-MS) was applied to the determination of five organophosphorous pesticides (OPPs) in water samples. The analytes included in this study were prophos, diazinon, chlorpyrifos methyl, fenchlorphos, and chlorpyrifos. The use of nonhalogenated solvents (cyclohexane, heptane, and octane) as extraction solvents was investigated using acetone, acetonitrile, or methanol, as dispersion solvents. The combination of less polar dispersion solvents (1-propanol and 2-propanol) and nonhalogenated extraction solvents was also studied in dispersive liquidliquid microextraction for the first time. Several experimental conditions were tested (nature and volume of extraction solvents, nature and volume of dispersion solvents, salting-out effect) and the corresponding enrichment factors and recoveries were evaluated. The best microextraction condition was obtained using 50 mu L of cyclohexane and 0.3 mL of 1-propanol. The detection and quantification limits were in the low ppt range, with values between 3.38.0 ng/L and 11.026.6 ng/L, respectively. Relative standard deviations were between 6.6 and 13.1% for a fortification level of 500 ng/L. At the same fortification level, the relative recoveries (RR) of Alvito's dam water, Judeu's river water, and well water samples were in the range of 50.397.1%.

• 出版日期2012-10