Determination of DNA Damage in vitro

作者：Dikomey E^*

来源：Nuklearmedizin, 2010, 49(6A): S64-S68.

• DNA double strand breaks
• cell inactivation
• chromosome aberration apoptosis G1 arrest DNA repair non homologous endjoining homologous recombination

Ionising irradiation acts primarily via induction of DNA damage among which double strand breaks are the most important lesions These lesions may lead to lethal chromosome aberrations which are the main reason for cell inactivation Double strand breaks can be re paired by several different mechanisms The regulation of these mechanisms appears be fairly different for normal and tumour cells Among different cell lines capacity of double strand break repair vanes by only few per cents and is known to be determined mostly by genetic factors Knowledge about double strand break repair mechanisms and their regulation is important for the optimal application of ionising irradiation in medicine

• 出版日期2010