Affinity membrane adsorbers for binding arginine-rich proteins

作者：Chenette Heather C S; Welsh James M; Husson Scott M^*

来源：Separation Science and Technology (Philadelphia), 2017, 52(2): 276-286.

DOI：10.1080/01496395.2016.1206934

• Arg-tag
• ATRP
• fusion protein
• membrane chromatography
• surface modification

Delivering protein chemotherapeutics into cancer cells is a challenge. Fusing the protein to an arginine-rich cell-penetrating peptide offers a possible solution. The goal of this work was to develop an affinity membrane for the purification of Arg-rich fusion proteins via capture chromatography. Membranes were prepared by grafting polymers bearing diethyl-4-aminobenzyl phosphonate (D4ABP) ligands from macroporous membrane supports. Incorporation of D4ABP was studied by infrared spectroscopy and energy dispersive spectroscopy. Protein-binding capacities of 3 mg lysozyme/mL were measured. While further studies are required to evaluate binding kinetics and Arg-selectivity, achieving higher protein-binding capacity is needed before investment in such studies.

• 出版日期2017