Development and validation of a fluorimetric method to determine curcumin in lipid and polymeric nanocapsule suspensions

作者：Mazzarino Leticia; Bellettini Ismael Casagrande; Minatti Edson; Lemos Senna Elenara^*

来源：Brazilian Journal of Pharmaceutical Sciences, 2010, 46(2): 219-226.

DOI：10.1590/s1984-82502010000200008

• Curcumin/determination
• Fluorimerric method/quantitative analysis
• Polymeric nanocapsules/evaluation
• Lipid nanocapsules/evaluation

A simple, rapid, and sensitive fluorimetrie method was developed and validated to quantify curcumin in lipid and polymeric nanocapsule suspensions, using acetonitrile as a solvent. The excitation and emission wavelengths were set at 397 am and 508 am, respectively. The calibration graph was linear from 0.1 to 0.6 mu g/mL with a correlation coefficient of 0.9982. The detection and quantitation limits were 0.03 and 0.10 mu g/mL, respectively. The validation results confirmed that the developed method is specific, linear, accurate, and precise for its intended use. The current method was successfully applied to the evaluation of curcumin content in lipid and polymeric nanocapsule suspensions during the early stage of formulation development.

• 出版日期2010-6