A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate

作者：Poon, Po Ying; Yue, Patrick Ying Kit; Wong, Ricky Ngok Shun^*

来源：Jove-Journal of Visualized Experiments, 2017, (121): e55411.

DOI：10.3791/55411

• Developmental Biology
• Issue 121
• cell migration assay
• mechanical wounder
• wound healing assay
• 96-well plate format
• cell monolayer
• adjustable

The cell migration/wounding assay is a commonly used method to study cell migration and other biological processes, such as angiogenesis and tumor metastasis. In this assay, cells are grown to form a confluent monolayer and a mechanical wound is created by scratching with a device. Then the migration rate of the cells towards the denuded area can be monitored by imaging. Our 8-channel mechanical wounder is designed to tackle most of the problems associated with the cell migration assay. Firstly, our wounder can be easily sterilized by autoclaving or with common disinfectants. Secondly, the individual adjustable pins allow even contact with the cell culture plate so that sharp and reproducible wounds can be created. Thirdly, the guiding bars on both sides of the wounder ensure consistent wounding position in each well. The use of disposable plastic pipette tips for wounding can further provide better handling of the wounder as well as to minimize cross-contamination. In conclusion, our cell wounder can provide researchers with a user friendly and reproducible device for performing the cell migration assay using the standard 96-well culture plate.

• 出版日期2017-3
• 单位香港浸会大学