ATP-sensitive inwardly inwardly rectifying potassium channel modulators alter cardiac function in honey bees

作者：O' Neal Scott T; Swale Daniel R; Blooniquist Jeffrey R; Anderson Troy D

来源：Journal of Insect Physiology, 2017, 99: 95-100.

DOI：10.1016/j.jinsphys.2017.04.005

• Honey bee
• Heart rate
• Ion channels
• K-ATP channels
• Tolbutamide
• Pinacidil

ATP-sensitive inwardly rectifying potassium (K-ATP) channels couple cellular metabolism to the membrane potential of the cell and play an important role in a variety of tissue types, including the insect dorsal vessel, making them a subject of interest not only for understanding invertebrate physiology, but also as a potential target for novel insecticides. Most of what is known about these ion channels is the result of work performed in mammalian systems, with insect studies being limited to only a few species and physiological systems. The goal of this study was to investigate the role that K-ATP channels play in regulating cardiac function in a model social insect, the honey bee (Apis mellifera), by examining the effects that modulators of these ion channels have on heart rate. Heart rate decreased in a concentration-dependent manner, relative to controls, with the application of the K-ATP channel antagonist tolbutamide and K-ATP channel blockers barium and magnesium, whereas heart rate increased with the application of a low concentration of the K-ATP channel agonist pinacidil, but decreased at higher concentrations. Furthermore, pretreatment with barium magnified the effects of tolbutamide treatment and eliminated the effects of pinacidil treatment at select concentrations. The data presented here confirm a role for K-ATP channels in the regulation of honey bee dorsal vessel contractions and provide insight into the underlying physiology that governs the regulation of bee cardiac function.

• 出版日期2017-6
• 单位Virginia Tech; 美国弗吉尼亚理工大学(Virginia Tech)