Microarray and pathway analysis of Differential Gene Expression between two kinds of prostate cancer cell lines sensitive and insensitive to docetaxel

作者：Zhu Sha; Gong Guangming; Oremo Judith Apondi; Zhen Minghui; Huang Tao; Tang Yue; Feng Guoqing

来源：Information Technology Journal, 2013, 12(24): 8281-8286.

DOI：10.3923/itj.2013.8281.8286

• Differential gene expressions
• Docetaxel
• Gene expression microarray
• Microarray data
• Oligonucleotide microarray
• Pathway analysis
• Prostate cancer cells
• Prostate cancers

Analyzing Differential Expression Gene (DEG) and identifying candidate gene in prostate cancer cell lines that were resistant to docetaxel, which might be used for the purposes of screening targeted therapy. Gene expression pro?les of the tumors from docetaxel treated mice were performed using Agilent human whole genomic oligonucleotide microarrays. The microarray data were validated and the differential expression genes were analyzed using IPA software. The prostate cancer cell lines PCS and LNCaP were sensitive to docetaxel but VcaP and CWR22 were resistant to docetaxel cell lines. It indicated that some genes up-regulated or down-regulated in docetaxel resistant cell lines may be candidate genes for targeted therapy, after evaluating by Gene Expression Microarray and signal pathway. The significantiy up-regulated genes including Bcl-11 A, EPHA4 and CX3CL1 may be the potential candidates for further investigation in docetaxel-resistant tumor cells.

• 出版日期2013
• 单位郑州大学