Amperometric determination of NADH based on a poly-Ni(II)-curcumin composite film modified glassy carbon electrode

作者：Ouyang, Xiaoqian; Ding, Yaping^*; Ba, Xi; Gu, Shuqing

来源：Analytical Methods, 2014, 6(18): 7496-7501.

DOI：10.1039/c4ay00884g

• ELECTROCATALYTIC OXIDATION
• COMPLEX
• BIOSENSOR
• CURCUMIN
• DEHYDROGENASE
• NANOPARTICLES
• METABOLISM
• NAD(+)
• CELL

A poly-Ni(II)-curcumin (curcumin: 1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) composite film modified electrode was prepared for amperometric determination of beta-nicotinamide adenine dinucleotide (NADH). Scanning electron microscopy and Energy Dispersive X-ray spectroscopy were used to examine the morphology and interface property of the Ni(II)-curcumin film. Under optimum conditions, the amperometric detection of NADH provided a wide linear detection range (0.3-300 mM), high sensitivity (230.02 mu A mM(-1) cm(-2)) and low limit of detection (LOD = 0.18 mu M, S/N = 3). Moreover, the modified electrode showed good stability and reproducibility, indicating that it can improve the analysis signal of NADH in a very convenient way.

• 出版日期2014
• 单位上海大学