A novel graphene oxide-based fluorescence assay for RNA endonuclease activity of mammalian Argonaute2 protein

作者：Li, Feng^*; Chen, Mingqin; Sun, Xinzhi; Wang, Xiuzhong; Li, Peng

来源：Sensors and Actuators B: Chemical , 2013, 182: 156-160.

DOI：10.1016/j.snb.2013.02.113

• Fluorescence
• Graphene oxide
• Argonaute2 protein
• RNA endonuclease activity

A novel graphene oxide (GO)-based amplified fluorescence assay for RNA endonuclease activity of mammalian Argonaute2 (Ago2) was developed. Owing to the extraordinarily high quenching efficiency of GO, the probe labeled with a fluorophore carboxyfluorescein exhibits minimal background fluorescence, while strong fluorescence intensity is observed with the addition of Ago2-miRNA complex due to the scission of the target mRNA sequences and the release of FAM-linked oligonucleotide fragments. Furthermore, Ago2-miRNA complex may be released to initiate another cycle of cleavage. The newly designed simple and cost-effective method exhibits high sensitivity with a detection limit of 1.7 nM (3 sigma) for Ago2 endonuclease activity.

• 出版日期2013-6
• 单位青岛科技大学; 青岛农业大学