Aqueous Two-Phase System as an Effective Tool for Purification of Phenolic Compounds from Fig Fruits (Ficus carica L.)

作者:Feng, Yun-Chao; Li, Wen-Liang; He, Fa-Mei; Kong, Tao-Tao; Huang, Xiao-Wei; Gao, Zhong-Hong; Lu, Nai-Hao; Li, Hai-Ling*
来源:Separation Science and Technology (Philadelphia), 2015, 50(12): 1785-1793.
DOI:10.1080/01496395.2015.1014054

摘要

Fig fruits (Ficus carica L.) are an excellent source of phenolic compounds. However, the presence of free sugars affects the quality of phenolic compounds. In this study, aqueous two-phase system was selected for purification of the phenolic compounds. The optimization of system parameters rendered conditions (ethanol 18% (w/w), K2HPO4 25% (w/w), temperature 10-30oC, and sample loading 3% (w/w)), under which more than 75% of phenolic compounds and 95% sugars were respectively recovered in the top and bottom phase. Moreover, some specific phenolic compounds content was enhanced and relatively high antioxidant activity was found after the purification process.