Ultrasensitive detection of clenbuterol by a covalent imprinted polymer as a biomimetic antibody

作者：Tang, Yiwei^*; Gao, Jingwen; Liu, Xiuying; Gao, Xue; Ma, Tao^*; Lu, Xiaonan^*; Li, Jianrong^*

来源：Food Chemistry, 2017, 228: 62-69.

DOI：10.1016/j.foodchem.2017.01.102

• Molecularly imprinted polymer
• Artificial antibody
• Biomimetic ELISA
• Clenbuterol

As an ideal biomimetic antibody, molecularly imprinted polymer (MIP) has shown great promise in immunoassays. Here, we developed a covalent imprinting method to prepare MIP artificial antibody towards clenbuterol on the well surface of a microtiter plate. Template molecule (clenbuterol) was splited by hydrolysis with functional monomer and removed by methanol/acetic acid solution, and then three-dimensional cavities were fabricated in the MIP, which can rebind template molecule via hydrogen bond interaction. Using this artificial antibody, we developed a novel biomimetic enzyme-linked immunosorbent assay (ELISA) with excellent sensitivity and specificity. The linear range for clenbuterol was 10(-5) to 1000 mu g L-1, with a detection limit of 10(-7) mu g L-1. Cross reactivity of this MIP artificial antibody to other four structural analogs was less than 0.93%. This method was applied to determine denbuterol in real samples with satisfactory result, suggesting a promising application of the biomimetic ELISA in food and environmental analysis.

• 出版日期2017-8-1
• 单位渤海大学