MicroRNA-1290 promotes asiatic acid-induced apoptosis by decreasing BCL2 protein level in A549 non-small cell lung carcinoma cells

作者：Kim Ki Bbeum; Kim Karam; Bae Seunghee; Choi Yeonghmin; Cha Hwa Jun; Kim Soo Yeon; Lee Jae Ho; Jeon So Hyeon; Jung Ho Jung; Ahn Kyu Joong; An In Sook; An Sungkwan^*

来源：Oncology Reports, 2014, 32(3): 1029-1036.

DOI：10.3892/or.2014.3319

• asiatic acid
• A549 cells
• microRNA
• BCL2
• apoptosis

Asiatic acid, a triterpenoid derived from Centella asiatica, is a putative anticancer agent in several types of cancer cells. Investigations of its biological role in negative regulation of cell growth have focused on the extent of induction of apoptosis in a cell-type-specific manner. In this study, we identified an important regulator of asiatic acid-induced cell death, microRNA (miR)-1290, which sensitizes cells to asiatic acid-induced cytotoxicity and negatively regulates BCL2 expression. Asiatic acid significantly upregulated miR-1290, and asiatic acid-induced cell death was shown to be dependent on miR-1290 activity. Molecular assays demonstrated that BCL2 mRNA is a direct target of miR-1290-mediated RNA interference. The results of functional studies suggest that miR-1290 suppresses cell viability and cell cycle progression. These data provide insight into miR-1290-mediated cellular mechanisms in asiatic acid-treated A549 non-small cell lung carcinoma cells.

• 出版日期2014-9