Alternative Splicing Regulates Targeting of Malate Dehydrogenase in Yarrowia lipolytica

作者：Kabran Philomene; Rossignol Tristan; Gaillardin Claude; Nicaud Jean Marc; Neuveglise Cecile^*

来源：DNA Research, 2012, 19(3): 231-244.

DOI：10.1093/dnares/dss007

• yeast
• TCA cycle
• glyoxylate cycle
• MDH2
• intron

Alternative pre-mRNA splicing is a major mechanism contributing to the proteome complexity of most eukaryotes, especially mammals. In less complex organisms, such as yeasts, the numbers of genes that contain introns are low and cases of alternative splicing (AS) with functional implications are rare. We report the first case of AS with functional consequences in the yeast Yarrowia lipolytica. The splicing pattern was found to govern the cellular localization of malate dehydrogenase, an enzyme of the central carbon metabolism. This ubiquitous enzyme is involved in the tricarboxylic acid cycle in mitochondria and in the glyoxylate cycle, which takes place in peroxisomes and the cytosol. In Saccharomyces cerevisiae, three genes encode three compartment-specific enzymes. In contrast, only two genes exist in Y. lipolytica. One gene (YIMDH1, YALI0D16753g) encodes a predicted mitochondrial protein, whereas the second gene (YIMDH2, YALI0E14190g) generates the cytosolic and peroxisomal forms through the alternative use of two 3'-splice sites in the second intron. Both splicing variants were detected in cDNA libraries obtained from cells grown under different conditions. Mutants expressing the individual YlMdh2p isoforms tagged with fluorescent proteins confirmed that they localized to either the cytosolic or the peroxisomal compartment.

• 出版日期2012-6