Molecular Analysis of Antimicrobial Resistance in Gram-Negative Bacteria Isolated from Fish Farms in Egypt

作者：Ishida Yojiro; Ahmed Ashraf M; Mahfouz Nadia B; Kimura Tomomi; El Khodery Sabry A; Moawad Amgad A; Shimamoto Tadashi^*

来源：Journal of Veterinary Medical Science, 2010, 72(6): 727-734.

DOI：10.1292/jvms.09-0538

• beta-lactamase
• environmental samples
• integron
• multidrug resistance
• public health

As little is known about antimicrobial resistance genes in fish farms, this study was conducted to monitor the incidence and prevalence of a wide range of antimicrobial resistance genes in Gram-negative bacteria isolated from water samples taken from fish farms in the northern part of Egypt. Ninety-one out of two hundred seventy-four (33.2%) non-repetitive isolates of Gram-negative bacteria showed multidrug resistance phenotypes and harbored at least one antimicrobial resistance gene. PCR and DNA sequencing results showed that 72 (26.3%) isolates contain tetracycline resistance genes and 19 (6.9%) isolates were positive for class 1 integrons with 12 different gene cassettes. The beta-lactamase-encoding genes were identified in 14(5.1%) isolates. The plasmid-mediated quinolone resistance genes, qnr and aac(6)-Ib-cr, were identified in 16(5.8%) and 3 (1.1%) isolates, respectively. Finally, the florphenicol resistance gene, floR, was identified in four (1.5%) isolates. To the best of our knowledge, this is the first report for molecular characterization of antimicrobial resistance in Gram-negative bacteria isolated from fish farms in Africa.

• 出版日期2010-6