Dual-tracer background subtraction approach for fluorescent molecular tomography

作者：Tichauer Kenneth M; Holt Robert W; El Ghussein Fadi; Davis Scott C; Samkoe Kimberley S; Gunn Jason R; Leblond Frederic; Pogue Brian W^*

来源：Journal of Biomedical Optics, 2013, 18(1): 016003.

DOI：10.1117/1.JBO.18.1.016003

• image reconstruction
• cancer
• targeted imaging agent
• small animal

Diffuse fluorescence tomography requires high contrast-to-background ratios to accurately reconstruct inclusions of interest. This is a problem when imaging the uptake of fluorescently labeled molecularly targeted tracers in tissue, which can result in high levels of heterogeneously distributed background uptake. We present a dual-tracer background subtraction approach, wherein signal from the uptake of an untargeted tracer is subtracted from targeted tracer signal prior to image reconstruction, resulting in maps of targeted tracer binding. The approach is demonstrated in simulations, a phantom study, and in a mouse glioma imaging study, demonstrating substantial improvement over conventional and homogenous background subtraction image reconstruction approaches. (c) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.18.1.016003]

• 出版日期2013-1