A Case Report Concerning Male Gametes Rescued from a Siamese Eld's Deer (Rucervus eldii siamensis): Post-Thawed Testicular and Epididymal Sperm Quality and Heterologous Zona Pellucida Binding Ability

作者：Thuwanut Paweena; Thongphakdee Ampika; Sommanustweechai Angkana; Siriaroonrat Boripat; Chatdarong Kaywalee^*

来源：Journal of Veterinary Medical Science, 2013, 75(1): 123-125.

DOI：10.1292/jvms.11-0491

• Siamese Eld's deer
• sperm collection post mortem
• sperm cryopreservation

In the present study, the quality of frozen-thawed epididymal and testicular sperm recovered from a Siamese Eld's deer was examined. The epididymal sperm quality was assessed in fresh, cold-stored at 4 degrees C and frozen-thawed samples. Zona binding ability of the frozen-thawed epididymal samples with Burmese Eld's deer oocytes was also evaluated. Testicular sperm extracted from tissues frozen at -80 or -196 degrees C for one month were examined for membrane and DNA integrity. Epididymal sperm retained their quality for up to 24 hr of cold storage at 4 degrees C. The percentages of sperm motility, intact membrane, intact acrosome and intact DNA were 30, 46.5, 27 and 89.5% in the frozen and thawed epididymal sperm, and the average ability to bind with oocytes was 92.5 +/- 64 sperm/oocytes. Around 70% of the sperm extracted from testicular tissues cryopreserved at -196 and -80 degrees C for one month showed an intact membrane. In conclusion, epididymal and testicular sperm survived for more than 13 hr post-mortem. Furthermore, cold storage at 4 degrees C and cryopreservation at -196 and -80 degrees C maintain the quality of epididymal and testicular sperm. This study represents a model for male gamete rescue in endangered Eld's deer.

• 出版日期2013-1