科研之友
微信
新浪微博
Facebook
分享链接
摘要
Potato starch waste, a chips industry effluent, was used for the production of industrially important enzymes by an amylolytic strain of lactic acid bacteria, isolated from pickled yam & identified as Lactococcus lactis. The strain was observed to co-produce alpha-amylase and beta-galactosidase. Potato starch waste was efficiently utilized (91.6%) along with high co-production of alpha-amylase and beta-galactosidase. Optimum culture conditions favouring maximum production of these two hydrolases in MRS medium containing 2% potato starch were temperature 55 degrees C, pH 7 and temperature 35 degrees C, pH 5 for alpha-amylase (2.54 U/mL) and beta-galactosidase (2.67 U/mL) respectively. In potato starch waste, Lactococcus lactis retained viability and could co-produce alpha-amylase (17.54 U/mL) and beta-galactosidase (25.35 U/mL) at a temperature of 45 degrees C and pH 6.5 within a period of 48 hrs. Thus, the study suggests a potential applicability of the isolate for the production of industrially significant hydrolases from a cheap agro industrial source.
- 出版日期2014-5
