Directing Assembly and Disassembly of 2D MoS2 Nanosheets with DNA for Drug Delivery

作者：Li Bang Lin; Setyawati Magdiel I; Chen Linye; Xie Jianping; Ariga Katsuhiko; Lim Chwee Teck; Garaj Slaven; Leong David Tai^*

来源：ACS Applied Materials & Interfaces, 2017, 9(18): 15286-15296.

DOI：10.1021/acsami.7b02529

• molybdenum disulfide
• DNA functionalization
• layer-by-layer assembly
• 3D cell culture
• targeted chemotherapy

Layer-by-layer (LbL) self-assembled stacked Testudo-like MoS2 superstructures carrying cancer drugs are formed from nanosheets controllably assembled with sequence-based DNA oligonucleotides. These superstructures can disassemble autonomously in response to cancer cells' heightened ATP metabolism. First, we functionalize MoS2 nanosheets (MoS2-NS) nanostructures with DNA oligonucleotides having thiol-terminated groups (DNA/MoS2-NS) via strong binding to sulfur atom defect vacancies on MoS2 surfaces. The driving force to assemble into a higher-order DNA/MoS2-NS superstructure is guided by a linker aptamer that induced interlayer assembly. In the presence of target ATP molecules, these multilayer superstructures disassembled as a consequence of stronger binding of ATP molecules with the linking aptamers. This design plays a dual role of protection and delivery by LbL stacked MoS2-NS similar in concept to a Greek Testudo. These superstructures present a protective armor-like shell of MoS2-NS, which still remains responsive to small and infiltrating ATP molecules diffusing through the protective MoS2NS, contributing to an enhanced stimuli-responsive drug release system for targeted chemotherapy.

• 出版日期2017-5-10