A radioresistant fraction of acute promyelocytic leukemia cells exhibit CD38 cell-surface antigen and mRNA expression

作者：Monzen Satoru^*; Chiba Mitsuru; Ueno Tatsuya; Morino Yuki; Terada Kenji; Yamaya Hiroki; Hosokawa Yoichiro

来源：Oncology Letters, 2018, 15(5): 6709-6714.

DOI：10.3892/ol.2018.8099

• acute promyelocytic leukemia cells
• cluster of differentiation 38
• radioresistant
• mRNA expression

In the present study, the cell viability and cluster of differentiation (CD) 38 mRNA expression were evaluated in radioresistant (Res)-HL60 acute promyelocytic leukemia (APL) cells. Cell viability in Res-HL60 cells was higher compared with wild-type HL60 cells, but did not differ between high and mid/low CD38 antigen expression groups in Res-HL60 cells. A higher expression of CD38 mRNA in Res-HL60 cells was observed, particularly in the CD38(high) cell subpopulation. Furthermore, the expression of CD38 mRNA was upregulated following exposure to X-irradiation. In contrast, the characteristic expression of CD45 and CCAAT/enhancer-binding protein a mRNA were not altered. These results suggest that the accumulation of CD38 protein in radioresistant APL cells, resulting from the constant expression of CD38 mRNA induced by X-irradiation, is a characteristic response of the radioresistant-surviving fraction; however, the accumulation of CD38 did not influence the extent of radioresistant behavior.

• 出版日期2018-5