A RAPID AND SIMPLE ARO GENOTYPE SCREENING METHOD USING A NOVEL B/O-2 VERSUS A/O-2 DISCRIMINATING NUCLEOTIDE SUBSTITUTION AT THE ABO HOCUS

作者：OLSSON ML; CHESTER MA

来源：Vox Sanguinis, 1995, 69(3): 242-247.

An ABO genotype screening method discriminating the common alleles A(1), A(2) B, O-1 and O-2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O-2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors, This technique is the most cost-efficient and informative ABO genotyping method reported to date.

• 出版日期1995