Amine functionalized nanodiamond promotes cellular adhesion, proliferation and neurite outgrowth

作者：Hopper A P^*; Dugan J M; Gill A A; Fox O J L; May P W; Haycock J W; Claeyssens F

来源：Biomedical Materials (Bristol), 2014, 9(4): 045009.

DOI：10.1088/1748-6041/9/4/045009

• nanodiamond
• Schwann cells
• neurons
• functionalization
• amine
• peripheral nerve

In this study, we report the production of amine functionalized nanodiamond. The amine functionalized nanodiamond forms a conformal monolayer on a negatively charged surface produced via plasma polymerization of acrylic acid. Nanodiamond terminated surfaces were studied as substrates for neuronal cell culture. NG108-15 neuroblastoma-glyoma hybrid cells were successfully cultured upon amine functionalized nanodiamond coated surfaces for between 1 and 7 d. Additionally, primary dorsal root ganglion (DRG) neurons and Schwann cells isolated from Wistar rats were also successfully cultured over a period of 21 d illustrating the potential of the coating for applications in the treatment of peripheral nerve injury.

• 出版日期2014-8