科研之友
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摘要
Context: Amaranthus spinosus Linn. (Amaranthaceae), commonly known as "spiny pigweed", is used both in the Indian traditional system and in folk medicine to treat diabetes. Objective: The present study evaluates the scientific basis of antidiabetic activity of chloroform fraction of methanol extract of A. spinosus and of an isolated constituent of A. spinosus. Materials and methods: HPLC analysis was performed to determine the purity and the amount of the constituent present in the plant extract. The yeast alpha-glucosidase inhibition technique was used to determine the antidiabetic activity of A. spinosus. Acarbose was used as a standard. An appropriate therapeutic approach for preventing diabetes mellitus and obesity is to retard the absorption of glucose by inhibition of alpha-glucosidase. Results: One novel fatty acid with strong alpha-glucosidase inhibitory activity -(14E, 18E, 22E, 26E) - methyl nonacosa-14, 18, 22, 26 tetraenoate [1] (IC50 value 6.52 mu M/mL) and beta-sitosterol [2] were purified. Compound 1 was found to be more potent than the methanol extract. HPLC quantative analysis revealed that 0.15% of compound 1 and 0.06% of compound 2 were present in the plant extract. Conclusion: This novel fatty acid can potentially be developed as a novel natural nutraceutical for the management of diabetes.
- 出版日期2015-4
