Developmental AMPA receptor subunit specificity during experience-driven synaptic plasticity in the rat barrel cortex

作者：Miyazaki Tomoyuki; Kunii Misako; Tada Hirobumi; Sano Akane; Kuroiwa Yoshiyuki; Goto Takahisa; Malinow Roberto; Takahashi Takuya^*

来源：Brain Research, 2012, 1435: 1-7.

DOI：10.1016/j.brainres.2011.11.033

• AMPA receptor
• Barrel
• Cortex
• Development
• Synapse
• Plasticity

During early postnatal brain development, experience-driven delivery of AMPA receptors to synapses participates in the initial organization of conical function. By combining virus-mediated in vivo gene delivery with in vitro whole cell recordings, we identified a subunit-specific developmental program of experience-driven AMPA receptor delivery to synapses in rat barrel cortex. We expressed green fluorescent protein (GFP)-tagged AMPA receptors (GFP-GluR1, or GFP-GluR4) into layer 2/3 pyramidal neurons at two distinct developmental periods, postnatal day (P)8-P10 and P12-P14. Two days after viral infection, acute brain slices were prepared, and synaptic transmission from layer 4 to layer 2/3 was analyzed by whole cell recordings. We found that whisker experience drives GluR4 but not GluR1 into these synapses early in postnatal development (P8-1310). However, at P12-14, GluR1 but not GluR4 is delivered into synapses by whisker experience. This precise developmental plan suggests unique plasticity properties endowed in different AMPA receptor subunits which shape the initial experience-driven organization of cortical function.

• 出版日期2012-1-30