Development of a pyrosequencing assay for rapid assessment of quinolone resistance in Acinetobacter baumannii isolates

作者：Deccache Yann; Irenge Leonid M; Savov Encho; Ariciuc Mihaela; Macovei Alexandra; Trifonova Angelina; Gergova Ivanka; Ambroise Jerome; Vanhoof Raymond; Gala Jean Luc^*

来源：Journal of Microbiological Methods, 2011, 86(1): 115-118.

DOI：10.1016/j.mimet.2011.04.007

• Acinetobacter baumannii
• Quinolone resistance
• gyrA
• parC
• Pyrosequencing

Rapid and reliable assessment of Acinetobacter baumannii resistance to quinolones was successfully achieved through pyrosequencing of the gyrA and parCquinolone-resistance determining regions. A strong correlation was found between quinolone resistance and mutations in gyrA codon 83 and/or in the parC gene (codons 80 or 84). Absence of QRDR mutations was associated with susceptibility to quinolones.

• 出版日期2011-7
• 单位中国人民解放军军事医学科学院