科研之友
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摘要
Objective: To evaluate sex selection of spermatozoa.
Design: A deoxyribonucleic acid (DNA) probe (pDP34) detecting distinguishable loci on both X and Y chromosome was used to validate the quinacrine-staining method that is often used for determination of the percentage of Y-bearing sperm. Sperm samples were centrifuged over Percoll to obtain samples with a high X:Y ratio according to the quinacrine-staining method. Controls (sperms before processing over Percoll) and processed sperms were subjected to DNA extraction and analysis with the DNA probe.
Results: The DNA analysis revealed a 1.0 X:Y ratio of the spermatozoa before and after Percoll separation.
Conclusion: We conclude that the quinacrine method is not suitable for evaluation of methods that claim to separate X and Y-bearing sperm.
- 出版日期1992-8
