Comprehensive Profiles of Human Milk Oligosaccharides Yield Highly Sensitive and Specific Markers for Determining Secretor Status in Lactating Mothers

作者：Totten Sarah M; Zivkovic Angela M; Wu Shuai; UyenThao Ngyuen; Freeman Samara L; Ruhaak L Renee; Darboe Momodou K; German J Bruce; Prentice Andrew M; Lebrilla Carlito B^*

来源：Journal of Proteome Research, 2012, 11(12): 6124-6133.

DOI：10.1021/pr300769g

• Lewis blood type
• secretor status
• human milk oligosaccharides
• nano LC-TOF/MS

Human milk oligosaccharides (HMOs), as an abundant and bioactive component of breast milk, work in many ways to promote the health of breast fed infants. The expression of HMOs has been shown to vary in accordance with Lewis blood type and secretor status, as women of different blood types differ in the expression of alpha 1,2 fucosyltransferase (FUT2) and alpha 1,3/4 fucosyltransferase (FUT3). In this study, HMOs were extracted from the milk of 60 women from The Gambia, Africa with various Lewis and secretor blood types. The HMOs were profiled using high resolution HPLC-Chip/TOF mass spectrometry. Notably, the amounts of fucosylation varied significantly between Le(a+b-) nonsecretors, Le(a-b+) and Le(a-b-) secretors, and Le(a-b-) nonsecretors. With higher frequency of expression of the recessive Lewis negative and nonsecretor phenotypes in West African populations, the HMO profiles of several milks from women of these phenotypes were examined, demonstrating decreased amounts of total oligosaccharide abundance and lower relative amounts of fucosylation. Also in this study, four specific fucosylated structures (2'FL, LNFP I, LDFT, and LNDFH I) were determined to be specific and sensitive glycan markers for rapidly determining secretor status without the need for serological testing.

• 出版日期2012-12