Application of novel magnetizable cellulose particles in the development of immunoradiometric assay for C-peptide

作者：Rasmi R R; Shenoy K Bhasker^*; Kadwad V B; Sarnaik Jayula; Somashekarappa H M

来源：Journal of Radioanalytical and Nuclear Chemistry, 2015, 304(3): 1115-1122.

DOI：10.1007/s10967-015-3925-8

• Magnetizable cellulose particles
• C-peptide
• Monoclonal antibodies
• Immunoradiometric assay
• Diabetes

The present study was oriented to develop, optimize and validate immunoradiometric assay for the measurement of C-peptide in human serum by employing magnetizable cellulose particles prepared by a novel method of wet grinding. Six commercial monoclonal antibodies raised against the different epitopes of C-peptide were evaluated for their compatibility as sandwich partners by "cross matching". The developed one step inclusive assay with best matched combination of sandwich complex, "magnetizable cellulose particles linked capture antibody-C-peptide-I-125 labelled detector antibody" had an analytical sensitivity of 0.16 ng/ml and standard range of 0.3-30 ng/ml covering required physiological and diagnostic range.

• 出版日期2015-6