An explant based-method for differentiating adipocytes from equine adipose tissue

作者：Suagee J K; Corl B A^*

来源：Equine Veterinary Journal, 2013, 45(1): 114-116.

DOI：10.1111/j.2042-3306.2012.00581.x

• horse
• adipose tissue
• mesenteric
• subcutaneous
• cell culture
• preadipocyte

Culturing adipocytes enables fine control of experimental conditions and helps minimise animal use. This report describes an explant-based method for isolating stromal-vascular cells from equine adipose tissue that enables use of small amounts of tissue. Subcutaneous and mesenteric adipose tissues were harvested post mortem and stromal-vascular cells grown from explants, prior to testing the capacity of several differentiation media to induce lipid droplet formation and increase transcript abundance of adipocyte markers. Inclusion of rosiglitazone at 1 and 5 mu mol/l concentrations, along with other media components, induced differentiation of cultured equine stromal-vascular cells derived from subcutaneous and mesenteric adipose tissues.

• 出版日期2013-1
• 单位Virginia Tech; 美国弗吉尼亚理工大学(Virginia Tech)