科研之友
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摘要
Recently, peach trees showing leaf rolling, little leaf, rosetting, yellowing, bronzing of foliage and tattered and shot-holed leaves symptoms were observed in peach growing areas in the central and north-western regions of Iran. Polymerase chain reaction (PCR) and nested PCR using phytoplasma universal primer pairs P1/Tint, R16F2/R2, PA2F/R and NPA2F/R were employed to detect phytoplasmas. The nested PCR assays detected phytoplasma infections in 51% of symptomatic peach trees in the major peach production areas in East Azerbaijan, Isfahan, ChaharMahal-O-Bakhtiari and Tehran provinces. Restriction fragment length polymorphism (RFLP) analyses of 485 bp fragments amplified using primer pair NPA2F/R in nested PCR revealed that the phytoplasmas associated with infected peaches were genetically different and they were distinct from phytoplasmas that have been associated with peach and almond witches'-broom diseases in the south of Iran. Sequence analyses of partial 16S rDNA and 16S-23S rDNA intergenic spacer regions demonstrated that 'Candidatus Phytoplasma aurantifolia', 'Ca. Phytoplasma solani' and 'Ca. Phytoplasma trifolii' are prevalent in peach growing areas in the central and north-western regions of Iran.
- 出版日期2010-2-10
