Lysosomal Membrane Permeabilization by Targeted Magnetic Nanoparticles in Alternating Magnetic Fields

作者：Domenech Maribella; Marrero Berrios Ileana; Torres Lugo Madeline; Rinaldi Carlos^*

来源：ACS Nano, 2013, 7(6): 5091-5101.

DOI：10.1021/nn4007048

• iron oxide
• magnetic nanoparticles
• epidermal growth factor
• epidermal growth factor receptor
• alternating magnetic field
• lysosomal membrane permeabilization
• lysosomal death pathways

Lysosomal death pathways are being explored as alternatives of overcoming cancer tumor resistance to traditional forms of treatment. Nanotechnologles that can selectively target and induce permeabilization of lysosomal compartments in cells could become powerful medical tools. Here we demonstrate that iron oxide magnetic nanoparticles (MNPs) targeted to the epidermal growth factor receptor (EGFR) can selectively Induce lysosomal membrane permeabilization (LMP) in cancer cells overexpressing the EGFR under the action of an alternating magnetic field (AMF). LMP was observed to correlate with the production of reactive oxygen species (ROS) and a decrease in tumor cell viability. Confocal microscopy images showed an Increase in the cytosolic activity of the lysosomal protease cathepsin B. These observations suggest the possibility of remotely triggering lysosomal death pathways In cancer cells through the administration of MNPs which target lysosomal internalization pathways and the application of AMFs.

• 出版日期2013-6