Diversity, relationships, and genetic fingerprinting of the Listada de Gandia eggplant landrace using genomic SSRs and EST-SSRs

作者：Munoz Falcon Julio E; Vilanova Santiago; Plazas Mariola; Prohens Jaime^*

来源：Scientia Horticulturae, 2011, 129(2): 238-246.

DOI：10.1016/j.scienta.2011.03.034

• EST-SSRs
• Fingerprinting
• Genomic SSRs
• Landraces
• Listada de Gandia
• Solanum melongena

Listada de Gandia is one of the most renowned Spanish eggplant (Solanum melongena L.) landraces. Assessing its genetic diversity and relationships, as well as devising tools for its identification, is of great relevance for the enhancement and protection of this landrace. Forty-two eggplant accessions, which included 25 Striped accessions, of which 19 were of the Listada type (six accessions of Listada de Gandia, eight of Other Spanish Listada, and five of Non-Spanish Listada) and six of the Other Non-Spanish Striped group, and 17 Non-Striped accessions were characterized with 17 genomic SSRs and 32 EST-SSRs. Genomic SSRs had, as a mean, a greater polymorphism and polymorphic information content (PIC) than EST-SSRs. Although Listada de Gandia proved to be genetically diverse, specific and universal alleles for two SSR markers were found for this landrace. All the Listada accessions cluster together in the multivariate PCoA and UPGMA phenograms performed, and are separated from the Other Non-Spanish Striped and Non-Striped accessions. Also, Listada de Gandia accessions were clearly differentiated from the Other Spanish Listada and Non-Spanish Listada accessions in these analyses. SSR markers revealed of great utility to obtain a specific fingerprint for the Listada de Gandia eggplant as well as to establish the uniqueness and distinctness of this landrace. This information will be very helpful for the enhancement and protection from imitation of Listada de Gandia, and contributes to support its potential recognition with a Protected Designation of Origin (PDO) status.

• 出版日期2011-6-10