Molecular detection of Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) in procyonids (Carnivora: Procyonidae) in Eastern Amazon, Brazil

作者：Magalhaes Matos Paulo Cesar; Cunha Santos Rafaelle; Silva Sousa Paulo Geovani; Sampaio Junior Francisco Dantas; Leite Barros Flavia de Nazare; Paixao Mourao Fabio Rodrigo; Lima Oliveira Wanessa Batista; Gabriel Aurea Martins; Monteiro Silvia Gonzalez; Goes Cavalcante Gustavo; Scofield Alessandra^*

来源：Ciencia Rural, 2016, 46(4): 663-668.

DOI：10.1590/0103-8478cr20150679

• Trypanosoma evansi
• PCR
• Procyonidae
• Eastern Amazon

The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapa and Para, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapa and Para. Whole blood samples were collected and kept at -20 degrees C for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive crabeating raccoons and captive and free-living coatis in Para State. No trypomastigote forms were observed in the blood smears. DNA from T. evansi was detected in P. cancrivorus and N. nasua in Para State, being this the first such report in P. cancrivorus.

• 出版日期2016-4
• 单位河北医科大学