Magnetic isotope and magnetic field effects on the DNA synthesis

作者：Buchachenko Anatoly L^*; Orlov Alexei P; Kuznetsov Dmitry A; Breslavskaya Natalia N

来源：Nucleic Acids Research, 2013, 41(17): 8300-8307.

DOI：10.1093/nar/gkt537

Magnetic isotope and magnetic field effects on the rate of DNA synthesis catalysed by polymerases beta with isotopic ions Mg-24(2+), Mg-25(2+) and Mg-26(2+) in the catalytic sites were detected. No difference in enzymatic activity was found between polymerases beta carrying Mg-24(2+) and Mg-26(2+) ions with spinless, non-magnetic nuclei Mg-24 and Mg-26. However, Mg-25(2+) ions with magnetic nucleus Mg-25 were shown to suppress enzymatic activity by two to three times with respect to the enzymatic activity of polymerases beta with Mg-24(2+) and Mg-26(2+) ions. Such an isotopic dependence directly indicates that in the DNA synthesis magnetic mass-independent isotope effect functions. Similar effect is exhibited by polymerases beta with Zn2+ ions carrying magnetic Zn-67 and non-magnetic Zn-64 nuclei, respectively. A new, ion-radical mechanism of the DNA synthesis is suggested to explain these effects. Magnetic field dependence of the magnesium-catalysed DNA synthesis is in a perfect agreement with the proposed ion-radical mechanism. It is pointed out that the magnetic isotope and magnetic field effects may be used for medicinal purposes (trans-cranial magnetic treatment of cognitive deceases, cell proliferation, control of the cancer cells, etc).

• 出版日期2013-9