Nitric Oxide and Carbon Monoxide Act as Inhibitory Neurotransmitters in the Longitudinal Muscle of C57BL/6J Mouse Distal Colon

作者：Hidaka Ayako; Azuma Yasu Taka; Nakajima Hidemitsu; Takeuchi Tadayoshi^*

来源：Journal of Pharmacological Sciences, 2010, 112(2): 231-241.

DOI：10.1254/jphs.09242FP

• mouse distal colon
• nonadrenergic noncholinergic (NANC) relaxation
• nitric oxide (NO)
• carbon oxide (CO)

The present study was designed to identify the inhibitory nuerotransmitters mediating nonadrenergic noncholinergic relaxation in the longitudinal msucle of C57/BL mouse distal colon. Relaxation induced by electrical field stimulation (EFS) was recorded isotonically in the presence of atropine and guanethidine. EFS-induced relaxation was inhibited by nitro-l-arginine(L-NNA) and Sn(IV) protoporphyrin dichloride IX (SnPP-IX), a nitric oxide (NO) and carbob monoxide (CO) synthase inhibitor, respectively. A combination of both inhibitors produced an additive effect. ODQ, a solubel guanylate cyclase inhibotor, inhibited EFS-induced relaxation. NOR-1, a NO donor, and carbon monoxide-releasing moelcule-2 (CORM-2), a CO donor, treatment relaxed teh distal colon and increased cyclic GMP content. The effects of NOR-1and CORM-2 were inhibited by ODQ.KT5823, a cyclic GMP-dependent protein kinase inhibitor, inhibited EFS-induced relaxation,a nd EFS-induced relaxation in the presence of H89 was further inhibited by L-NNA. These results suggested that NO and CO function as inhibitory neurotransmitters in the longitudinal muscle of C57BL mouse distal colon.

• 出版日期2010-2