Embryonic ectoderm development protein is regulated by microRNAs in human neural tube defects

作者：Song, Pei-Pei; Hu, Yi; Liu, Chun-Mei; Yan, Mu-Ju; Song, Ge; Cui, Yi; Xia, Hong-Fei^*; Ma, Xu

来源：American Journal of Obstetrics and Gynecology, 2011, 204(6): 544.e9.

DOI：10.1016/j.ajog.2011.01.045

• embryonic ectoderm development
• microribonucleic acid
• neural tube defects
• polycomb proteins

OBJECTIVE: The objective of the study was to investigate the expression and regulation of polycomb group (PcG) proteins in human neural tube defects (NTDs).
STUDY DESIGN: PcG proteins in human NTD fetuses and age-matched controls were detected by Western blot. The relation between PcG proteins and microribonucleic acids was predicted and confirmed by the bioinformatics method, real-time polymerase chain reaction (PCR), dual-luciferase activity assay, and Western blot. The trimethyl condition of histone H3 Lys27 (H3K27) was detected by immunohistochemical and immunofluorescence.
RESULTS: Embryonic ectoderm development protein (EED) was differentially detected in placenta, cerebral cortex, and spinal cord from NTDs and age-matched controls. MiR-30b can interact with 3'-untranslated region (UTR) of Eed and regulate endogenous EED expression in neural tissues. In addition, we found an inverse relationship between the miR-30b expression and the amount of trimethyl H3K27.
CONCLUSION: Differential expression of EED exists in the nerves system in human NTDs and that is regulated by miR-30b.

• 出版日期2011-6
• 单位中国医学科学院北京协和医院; 国家卫生计生委科学技术研究所