An ultrasensitive fluorescence aptasensor for carcino-embryonic antigen detection based on fluorescence resonance energy transfer from upconversion phosphors to Au nanoparticles

作者：Li, Xiang-Hui; Sun, Wei-Ming; Wu, Juan; Gao, Yao; Chen, Jing-Hua; Chen, Min^*; Ou, Qi-Shui^*

来源：Analytical Methods, 2018, 10(13): 1552-1559.

DOI：10.1039/c7ay02803b

• SENSITIVE DETECTION
• QUANTUM DOTS
• GOLD NANOPARTICLES
• TRANSFER BIOSENSOR
• GRAPHENE OXIDE
• HUMAN SERUM
• IONS
• APTAMERS
• SURFACE
• CANCER

A novel fluorescence resonance energy transfer (FRET)-based aptasensor was proposed for carcino-embryonic antigen (CEA) detection utilizing NaYF4:Yb3+, Er3+ upconversion nanoparticles (UCNPs) and gold nanoparticles (Au-NPs) as the energy donor and acceptor, respectively. The FRET process occurs when the single-stranded DNA modified UCNPs and Au-NPs are linked together by the CEA aptamer, which leads to the fluorescence quenching of UCNPs. However, the presence of CEA splits the above fluorescence-suppressed Au-NPs-aptamer-UCNPs sandwich complex, resulting in the fluorescence recovery of UCNPs. The recovery of fluorescence intensity is linearly correlated to the concentration of CEA in the range of 0.05-2.0 ng mL(-1), enabling the detection and quantification of CEA. Such a sandwich-type FRET-based assay possesses a low limit of detection (0.02 ng mL(-1)) and satisfactory selectivity and reproducibility, indicating that this biosensor is applicable for the trace detection of the CEA protein.

• 出版日期2018-4-7
• 单位福建医科大学