Decreased level of recent thymic emigrants in CD4+ and CD8+ T cells from CML patients

作者：Li, Yangqiu^*; Geng, Suxia; Yin, Qingsong; Chen, Shaohua; Yang, Lijian; Wu, Xiuli; Li, Bo; Du, Xin; Schmidt, Christian A.; Przybylski, Grzegorz K.

来源：Journal of Translational Medicine, 2010, 8: 47.

DOI：10.1186/1479-5876-8-47

• CHRONIC LYMPHOCYTIC-LEUKEMIA
• RECEPTOR EXCISION CIRCLES
• CLONAL EXPANSIONS
• MYELOID-LEUKEMIA
• ZETA-CHAIN
• TRANSPLANTATION
• REPERTOIRE
• OUTPUT
• MALIGNANCIES
• SUBSETS

Background: T-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. Based on our previous finding, to further characterize the immune status, T cell proliferative history was analyzed in CD4+ and CD8+ T cells from chronic myeloid leukemia (CML) patients.
Methods: Quantitative analysis of delta Rec-psi J alpha signal joint T cell receptor excision circles (sjTRECs) was performed in PBMCs, CD3+, CD4+ and CD8+ T cells by real-time PCR, and the analysis of 23 TRBV-D1 sjTRECs was performed by seminested PCR. Forty eight CML cases in chronic phase (CML-CP) were selected for this study and 17 healthy individuals served as controls.
Results: The levels of delta Rec-psi J alpha sjTRECs in PBMCs, CD3+, CD4+, and CD8+ T cells were significantly decreased in CML patients, compared with control groups. Moreover, the numbers of detectable TRBV subfamily sjTRECs, as well as the frequency of particular TRBV-BD1 sjTRECs in patients with CML were significantly lower than those from healthy individuals.
Conclusions: We observed decreased levels of recent thymic emigrants in CD4+ and CD8+ T cells that may underlay the persistent immunodeficiency in CML patients.

• 出版日期2010-5-14
• 单位广东省人民医院; 暨南大学