A novel benzimidazole and other constituents with antiproliferative and antioxidant properties from Thymelaea microphylla Coss. et Dur

作者：Noman Labib; Oke Altuntas Feyza^*; Zellagui Amar; Yaglioglu Ayse Sahin; Demirtas Ibrahim^*; Cardoso Susana M; Akkal Salah; Gherraf Noureddine; Rhouati Salah

来源：Natural Product Research, 2017, 31(17): 2032-2041.

DOI：10.1080/14786419.2016.1274888

• Thymelaea microphylla
• microphybenzimidazole
• antiproliferative activity
• HeLa
• C6
• antioxidant activity

A new compound (microphybenzimidazole, 7) along with the six known compounds matairesinol (1), prestegane B (2), umbelliferone (3), daphnoretin (4), microphynolide A (5) and microphynolide B (6) were isolated from Thymelaea microphylla. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. Compounds 2 and 4, as well as three fractions (F6, F6-C5, and F6-W42) obtained from a 50% (v:v) CH2Cl2:MeOH extract exhibited a selective activity against rat brain glioma cells (C6). Moreover, compound 1 and other fractions obtained from 50% (v:v) CH2Cl2:MeOH and 70% (v:v) MeOH:H2O extracts exhibited dose- and time-dependent effects on human cervical cancer cell (HeLa), as measured by xCELLigence assay. Compound 2 (IC50=14.0 +/- 0.2g/mL) and fraction F5 (IC50=12.4 +/- 0.1g/mL) showed higher radical scavenging ability than the synthetic agent butylated hydroxytoluene (BHT, IC50=22.7 +/- 0.6g/mL)

• 出版日期2017