High-throughput proteomics of breast carcinoma cells: a focus on FTICR-MS

作者：Umar Arzu^*; Jaremko Malgorzata; Burgers Peter C; Luider Theo M; Foekens John A; Pasa Tolic Ljiljana

来源：Expert Review of Proteomics, 2008, 5(3): 445-455.

DOI：10.1586/14789450.5.3.445

• accurate mass and time tag
• biomarker discovery
• breast cancer
• Fourier transform ion cyclotron resonance
• high throughput
• laser-capture microdissection
• MALDI
• nanoscale liquid chromatography
• protein array

Discovery of better biomarkers for diagnosis, prognosis and therapy-response prediction is the most critical task of a scientific quest aimed at developing novel, tailormade therapies for patients with cancer. Consequently, a proteome-wide analysis, in addition to genomic studies, is an absolute requirement for a complete functional understanding of tumor biology. Ultra-sensitive, high-performance Fourier-transform ion-cyclotron resonance (FTICR) mass spectrometry (MS) currently holds an important role in fulfilling the demands of biomarker discovery. In this review, we describe the applicability of FTICR-MS for breast cancer proteomics, particularly for the analysis of complex protein mixtures obtained from a limited number of cells typically available from clinical specimens.

• 出版日期2008-6