Analysis of nicotinic acetylcholine receptor subunits in the cochlea of the mouse

作者：Drescher DG^*; Khan KM; Green GE; Morley BJ; Beisel KW; Kaul H; Gordon D; Gupta AK; Drescher MJ; Barretto RL

来源：Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology, 1995, 112(3): 267-273.

DOI：10.1016/0742-8413(95)02020-9

• acetylcholine
• cochlea
• nicotinic receptors
• nucleotide sequencing
• PCR

The present study was designed to catalogue and compare nicotinic receptor subunit messages in the mammalian cochlea, Fourteen nicotinic acetylcholine receptor subunit messages were examined by polymerase chain reaction (PCR) analysis and nucleotide sequencing, Total RNA was extracted from the auditory organs of 14- to 18-day-old CBA(J) mice, and mRNA was purified using oligo-dT cellulose. After reverse transcription, resulting cDNA was amplified by PCR with the use of primers specific for the nucleotide sequences representing the following nicotinic receptor subunits: muscle types alpha 1, beta 1, gamma, delta and epsilon neuronal types alpha 2,3,4,5,6,7 and beta 2,3,4. cDNA from cochlear tissue corresponding to the muscle-type receptor subunit pi and to neuronal-type receptor subunits alpha 2,4,5,6 and beta 2,3 was amplified, whereas cDNA for muscle types alpha 1, gamma, delta, and epsilon and neuronal types alpha 3,7 and beta 4 was not. All PCR products were homologous in nucleotide sequence to the corresponding reference cDNAs from which the primers were designed, The current results indicate that nicotinic acetylcholine receptor (nAChR) subunits that are similar or identical to the stated muscle and neuronal types are expressed in the murine cochlea. The presence of messages corresponding to the muscle type pi and neuronal-type nAChR subunits may be correlated with the atypical cholinergic response of cochlear hair cells to agonists and antagonists.

• 出版日期1995-11