ALBUMIN REPERFUSION DECREASES INTESTINAL APOPTOSIS IN A RAT MODEL OF TRAUMA/HAEMORRHAGIC SHOCK VIA INHIBITION OF P38 MITOGEN-ACTIVATED PROTEIN KINASE

作者：Quan, Sheng; Chen, Yijie; Zhang, Feng; Zhang, Lihao; Wang, Chaohui; Yang, Zhangjie; Xia, Ziqi; Wu, Tianming; Xia, Xiaoping^*

来源：Acta Medica Mediterranea, 2015, 31(1): 167-172.

• Trauma/hemorrhagic shock (T/HS)
• albumin
• apoptosis
• mitogen-activated protein kinases (MAPKs)
• nuclear factor kappa B (NF-kappa B).

Aims: To determine the effects of albumin resuscitation on intestinal injury and apoptosis in a trauma/haemorrhagic shock (T/HS) rat model. @@@ Materials and methods: Studies were performed on an in vivo T/HS model in spontaneously breathing rats. Rats were subjected to femur fracture, ischemia for 30 min, and reperfusion for 20 min with Ringer's lactate solution (n=12) or 50 g/L albumin (n=12), and the intestines were resected 24 h later. Sham controls (n=12) received anaesthesia without T/HS or reperfusion. @@@ Results: Albumin reperfusion markedly decreased the number of positive apoptotic cells, 4.3 +/- 2.5% vs. Ringer's solution reperfusion group 22.0 +/- 7.4%, p<0.01. Semi-quantitative scoring of p38 mitogen-activated protein kinase (MAPK) in immunohistochemically stained jejunum revealed albumin reperfusion (1.8 +/- 0.6%) significantly attenuated expression of phosphorylated p38 MAPK 24 h after T/HS compared with Ringer's solution reperfusion (4.8 +/- 1.1%, p<0.01). NF-kappa B upregulation was also inhibited in the albumin reperfusion group (2.3 +/- 0.5%) compared with the Ringer's solution reperfusion group (5 +/- 0.8%, p<0.05). @@@ Conclusion: Resuscitation with albumin inhibits T/HS-induced apoptosis in the intestine via the reduced activation of the p38 MAPK signal transduction pathway and NF-kappa B signaling.

• 出版日期2015
• 单位浙江大学城市学院; 浙江大学